Reconstruction of ancient chromosomes offers insight into mammalian evolution
What if researchers could go back in time 105 million years and accurately sequence the chromosomes of the first placental mammal? What would it reveal about evolution and modern mammals, including humans?
Researchers from the 91做厙 in the UK collaborated with academics from University of California, Davis (UC Davis), Carnegie Mellon University in Pittsburgh, Konkuk University in Seoul and the University of Illinois at Urbana–Champaign, to go back in time by computationally recreating the chromosomes of the first eutherian mammal, the long-extinct shrew-like ancestor of all placental mammals.
The findings are published in Proceedings of the National Academy of Sciences, and have broad implications for understanding how chromosomal rearrangements over millions of years may contribute to human diseases, such as cancer.
Harris Lewin, a lead author of the study and a professor of evolution and ecology at UC Davis, said: “The revolution in DNA sequencing has provided us with enough chromosome-scale genome assemblies to permit the computational reconstruction of the eutherian ancestor, as well as other key ancestors along the lineage leading to modern humans. We now understand the major steps of chromosomal evolution that led to the genome organization of more than half the existing orders of mammals. These studies will allow us to determine the role of chromosome rearrangements in the formation of new mammal species and how such rearrangements result in adaptive changes that are specific to the different mammalian lineages.
“By gaining a better understanding of the relationship between evolutionary breakpoints and cancer breakpoints, the essential molecular features of chromosomes that lead to their instability can be revealed. Our studies can be extended to the early detection of cancer by identifying diagnostic chromosome rearrangements in humans and other animals, and possibly novel targets for personalized therapy.”
Descrambling chromosomes
To recreate the chromosomes of these ancient relatives, the team began with the sequenced genomes of 19 existing placental mammals — all eutherian descendants — including human, goat, dog, orangutan, cattle, mouse and chimpanzee.
The researchers then used a new algorithm they developed called DESCHRAMBLER which computed (“descrambled”) the most likely order and orientation of 2,404 chromosome fragments that were common among the 19 placental mammals’ genomes.
Jian Ma, the study’s co-senior author and an associate professor of computational biology at Carnegie Mellon University, said: “It is the largest and most comprehensive such analysis performed to date, and DESCHRAMBLER was shown to produce highly accurate reconstructions using data simulation.”
In addition to the eutherian ancestor, reconstructions were made for the six other ancestral genomes on the human evolutionary tree: boreoeutherian, euarchontoglires, simian (primates), catarrhini (Old World monkeys), great apes and human-chimpanzee. The reconstructions give a detailed picture of the various chromosomal changes that have occurred over the 105 million years between the first mammal and Homo sapiens.
Rates of Evolution Vary
One discovery is that the first eutherian ancestor likely had 42 chromosomes, four less than humans. Researchers identified 162 chromosomal breakpoints – locations where a chromosome broke open, allowing for rearrangements — between the eutherian ancestor and the formation of humans as a species.
